毛琼丽¹，李先芝^1，2*，严 玲¹，刘 洋¹，胡 杨¹，石 豪¹，朱 艳¹，杨 峰¹，陈彦和¹

（1.劲牌有限公司，湖北大冶 435100；

2.中药保健食品质量与安全湖北省重点实验室，湖北大冶 435100）

摘要：建立液相色谱-质谱联用法测定酒类产品中10种人工合成甜味剂（安赛蜜、甜蜜素、糖精钠、三氯蔗糖、阿斯巴甜、阿力甜、纽甜、甜菊糖苷、柚皮苷二氢查尔酮、新陈皮苷二氢查尔酮）的含量分析方法。液相色谱-质谱在负离子模式下，通过多反应监测离子模式方式进行检测。10种人工合成甜味剂在工作曲线范围内线性关系良好，相关系数均大于0.999 0，方法的定量限在0.35～12.0 μg/L之间，在3个不同浓度水平加标时，白酒产品中平均回收率93.16%～101.09%。结果表明该方法准确，灵敏度高，结果稳定可靠，可用于酒类产品中10种甜味剂的检测。

关键词：甜味剂；液相色谱-质谱联用；酒类产品；新橙皮甙二氢查尔酮；柚皮苷二氢查尔酮

中图分类号：TS207.3   文献标识码：A   文章编号：1674-506X（2021）06-0113-0007

Simultaneous Determination of 10 Sweeteners in Alcoholic Drinks by HPLC-MS

MAO Qiong-li¹, LI Xian-zhi^1，2*, YAN Ling¹, LIU Yang¹, HU Yang¹, SHI Hao¹, ZHU Yan¹, YANG Feng¹, CHEN Yan-he¹

（1.Jing Brand Co., Ltd., Daye Hubei 435100, China;

2.Hubei Provincial Key Lab for Quality and Safety of Traditional Chinese Medicine Health Food, Daye Hubei 435100, China）

Abstract：In this study, the content of 10 sweeteners in alcoholic drinks was determined simultaneously by liquid chromatography-mass spectrometry (LC-MS), including acesamel, cysalin, sodium saccharin, sucralose, aspartame, alitame, neotame, stevia glycoside, naringin dihydrochalcone and neocerperidin dihydrochalcone. Liquid chromatography-mass spectrometry (LC-MS) was used in the negative ion mode, and the detection was carried out in the multiple reaction monitoring ion mode. The linearity of the 10 synthetic sweeteners in the calibration curve range is good, the correlation coefficients are all greater than 0.999 0. The limits of quantitation were 0.35～12.0 μg/L. At three different concentration levels, the average recoveries of 10 compounds were between 93.16% and 101.09% in liquor. The method has a high accuracy and high selectivity, the result is stable, sensitive and reliable, it could be used for the detection of 10 kinds of sweeteners in liquor.

Keywords：sweeteners; liquid chromatography-mass spectrometry (LC-MS); alcoholic drinks; naringin dihydrochalcone; neocerperidin dihydrochalcone

doi：10.3969/j.issn.1674-506X.2021.06-019

收稿日期：2021-04-26

基金项目：湖北省重点研发计划（2020BBA050）

作者简介：毛琼丽（1982-），女，本科，工程师。研究方向：产品质量控制。

*通信作者

引文格式：毛琼丽,李先芝,严玲,等.酒类产品中10种甜味剂含量检测方法[J].食品与发酵科技,2021,57(6):113-119.